ʻUniconazolehe triazolemea hoʻoponopono ulu mea kanui hoʻohana nui ʻia e hoʻoponopono i ke kiʻekiʻe o ka mea kanu a pale i ka ulu nui ʻana o nā hua kanu. Eia nō naʻe, ʻaʻole maopopo loa ke ʻano molekala e kāohi ai ka uniconazole i ka hoʻolōʻihi ʻana o ka hypocotyl hua kanu, a he kakaikahi wale nō nā haʻawina e hoʻohui i ka ʻikepili transcriptome a me ka metabolome e noiʻi i ke ʻano o ka hoʻolōʻihi ʻana o ka hypocotyl. Maanei, ua ʻike mākou ua kāohi nui ka uniconazole i ka hoʻolōʻihi ʻana o ka hypocotyl i nā hua kanu kāpeti pua Kina. ʻO ka mea hoihoi, ma muli o ka loiloi transcriptome a me ka metabolome i hui pū ʻia, ua ʻike mākou ua hoʻopilikia nui ka uniconazole i ke ala "phenylpropanoid biosynthesis". Ma kēia ala, hoʻokahi wale nō gene o ka ʻohana gene regulatory enzyme, ʻo BrPAL4, ka mea i komo i ka biosynthesis lignin, ua hoʻohaʻahaʻa nui ʻia. Eia kekahi, ua hōʻike nā assays yeast hoʻokahi-hybrid a me ʻelua-hybrid e hiki iā BrbZIP39 ke hoʻopaʻa pololei i ka ʻāpana hoʻolaha o BrPAL4 a hoʻāla i kāna transcription. Ua hōʻoia hou ka ʻōnaehana hoʻopau gene i hoʻokomo ʻia e ka virus e hiki iā BrbZIP39 ke hoʻoponopono maikaʻi i ka hoʻolōʻihi ʻana o ka hypocotyl o ka kāpeti Kina a me ka synthesis hypocotyl lignin. Hāʻawi nā hopena o kēia noiʻi i nā ʻike hou i loko o ke ʻano hoʻoponopono molekala o cloconazole i ka pale ʻana i ka hoʻolōʻihi ʻana o ka hypocotyl o ka kāpeti Kina. Ua hōʻoia ʻia no ka manawa mua ua hōʻemi ka cloconazole i ka nui o ka lignin ma ka pale ʻana i ka synthesis phenylpropanoid i hoʻopili ʻia e ka module BrbZIP39-BrPAL4, a laila e alakaʻi ana i ka dwarfing hypocotyl i nā mea kanu kāpeti Kina.
No ka genus Brassica ka kāpeti Kina (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee) a he mea kanu cruciferous makahiki kaulana i ulu nui ʻia ma koʻu ʻāina (Wang et al., 2022; Yue et al., 2022). I nā makahiki i hala iho nei, ua hoʻomau ka hoʻonui ʻia ʻana o ka nui o ka hana ʻana o ka kāpeti Kina, a ua loli ke ʻano mahiʻai mai ka lūlū pololei kuʻuna i ka mahi kanu ʻana a me ka hoʻoili ʻana. Eia nō naʻe, i ke kaʻina hana o ka mahi kanu ʻana a me ka hoʻoili ʻana, ʻo ka ulu nui ʻana o ka hypocotyl e hoʻopuka i nā mea kanu wāwae, e hopena ana i ka maikaʻi ʻole o ka mea kanu. No laila, ʻo ka kaohi ʻana i ka ulu nui ʻana o ka hypocotyl he pilikia koʻikoʻi ia i ka mahi kanu ʻana a me ka hoʻoili ʻana o ka kāpeti Kina. I kēia manawa, he kakaikahi nā haʻawina e hoʻohui ana i ka ʻikepili transcriptomics a me ka metabolomics e ʻimi i ke ʻano o ka hoʻolōʻihi ʻana o ka hypocotyl. ʻAʻole i aʻo ʻia ke ʻano molekala e hoʻoponopono ai ka chlorantazole i ka hoʻonui ʻana o ka hypocotyl i loko o ka kāpeti Kina. Ua manaʻo mākou e ʻike i nā genes a me nā ala molekala e pane ana i ka uniconazole-induced hypocotyl dwarfing i loko o ka kāpeti Kina. Ma ka hoʻohana ʻana i nā loiloi transcriptome a me metabolomic, a me ka loiloi yeast one-hybrid, dual luciferase assay, a me ka virus-induced gene silencing (VIGS) assay, ua ʻike mākou hiki i ka uniconazole ke hoʻoulu i ka hypocotyl dwarfing i loko o ka kāpeti Kina ma ke kāohi ʻana i ka lignin biosynthesis i loko o nā mea kanu kāpeti Kina. Hāʻawi kā mākou mau hopena i nā ʻike hou i loko o ke ʻano hoʻoponopono molekala e kāohi ai ka uniconazole i ka elongation hypocotyl i loko o ka kāpeti Kina ma o ke kāohi ʻana i ka phenylpropanoid biosynthesis i hoʻopili ʻia e ka module BrbZIP39-BrPAL4. Hiki i kēia mau hopena ke loaʻa nā hopena kūpono koʻikoʻi no ka hoʻomaikaʻi ʻana i ka maikaʻi o nā mea kanu kalepa a me ka hāʻawi ʻana i ka hōʻoia ʻana i ka hua a me ka maikaʻi o nā mea kanu.
Ua hoʻokomo ʻia ka BrbZIP39 ORF piha i loko o ka pGreenll 62-SK e hana i ka effector, a ua hoʻohui ʻia ka ʻāpana hoʻolaha BrPAL4 i ka gene reporter pGreenll 0800 luciferase (LUC) e hana i ka gene reporter. Ua hoʻololi pū ʻia nā vectors gene effector a me reporter i nā lau paka (Nicotiana benthamiana).
No ka hoʻākāka ʻana i nā pilina o nā metabolites a me nā genes, ua hana mākou i kahi loiloi metabolome a me ka transcriptome hui. Ua hōʻike ka loiloi hoʻonui ala KEGG ua hoʻonui like ʻia nā DEG a me nā DAM i 33 mau ala KEGG (Kiʻi 5A). I waena o lākou, ʻo ke ala "phenylpropanoid biosynthesis" ka mea i hoʻonui nui ʻia; ʻo ke ala "photosynthetic carbon fixation", ke ala "flavonoid biosynthesis", ke ala "pentose-glucuronic acid interconversion", ke ala "tryptophan metabolism", a me ke ala "starch-sucrose metabolism". Ua hōʻike ka palapala ʻāina hoʻohui wela (Kiʻi 5B) ua māhele ʻia nā DAM e pili ana me nā DEG i kekahi mau māhele, a ʻo nā flavonoids ka māhele nui loa, e hōʻike ana ua hoʻokani ke ala "phenylpropanoid biosynthesis" i kahi kuleana koʻikoʻi i ka hypocotyl dwarfism.
Ke hōʻike nei nā mea kākau ua hana ʻia ka noiʻi me ka loaʻa ʻole o kekahi pilina kālepa a kālā paha i hiki ke wehewehe ʻia he hakakā hoihoi.
ʻO nā manaʻo a pau i hōʻike ʻia ma kēia ʻatikala, ʻo ia wale nō nā manaʻo o ka mea kākau a ʻaʻole pono e hōʻike i nā manaʻo o nā hui pili, nā mea hoʻopuka, nā mea hoʻoponopono, a i ʻole nā mea loiloi. ʻAʻole i hōʻoia ʻia a ʻae ʻia paha e ka mea hoʻopuka nā huahana i loiloi ʻia ma kēia ʻatikala a i ʻole nā koi i hana ʻia e kā lākou mea hana.
Ka manawa hoʻouna: Malaki-24-2025