Uniconazolehe triazolemea hoʻoponopono ului hoʻohana nui ʻia e hoʻoponopono i ke kiʻekiʻe o nā mea kanu a pale i ka ulu nui ʻana o ka hua. Eia nō naʻe, ʻaʻole maopopo loa ke ʻano molekala kahi uniconazole e kāohi ai i ka elongation hypocotyl seedling, a he mau haʻawina wale nō e hoʻohui i ka ʻikepili transcriptome a me ka metabolome e noiʻi i ke ʻano o ka elongation hypocotyl. Ma ʻaneʻi, ua ʻike mākou ua kāohi nui ka uniconazole i ka hoʻolōʻihi ʻana o ka hypocotyl i nā mea kanu pua pua Kina. ʻO ka mea e mahalo ai, ma muli o ka transcriptome hui pū ʻia a me ka nānā ʻana i ka metabolome, ua ʻike mākou ua pili nui ka uniconazole i ke ala "phenylpropanoid biosynthesis". Ma kēia ala, hoʻokahi wale nō gene o ka ʻohana gene regulatory gene, BrPAL4, i komo i ka biosynthesis lignin, i hoʻohaʻahaʻa nui ʻia. Eia kekahi, ua hōʻike ʻia nā hōʻike hū hoʻokahi-hybrid a me ʻelua-hybrid assays hiki iā BrbZIP39 ke hoʻopaʻa pololei i ka wahi hoʻolaha o BrPAL4 a hoʻāla i kāna transcription. Ua hōʻoia hou ka ʻōnaehana silencing gene i hoʻokomo ʻia i ka maʻi i hiki iā BrbZIP39 ke hoʻoponopono maikaʻi i ka elongation hypocotyl o ke kāpena Kina a me ka synthesis hypocotyl lignin. Hāʻawi nā hopena o kēia haʻawina i nā ʻike hou i ke ʻano hoʻoponopono molekala o cloconazole i ka pale ʻana i ka hypocotyl elongation o ke kāpena Kina. Ua hōʻoia ʻia no ka manawa mua ua hoʻemi ka cloconazole i ka lignin ma o ke kāohi ʻana i ka synthesis phenylpropanoid i hoʻopili ʻia e BrbZIP39-BrPAL4 module, a laila e alakaʻi ai i ka hypocotyl dwarfing i nā kumulāʻau kāpeti Kina.
No ka genus Brassica ka kāpeti Pākē (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee) a he mea kanu cruciferous makahiki kaulana i ulu nui ʻia ma koʻu ʻāina (Wang et al., 2022; Yue et al., 2022). I kēia mau makahiki i hala iho nei, ua hoʻomau ka hoʻonui ʻia ʻana o ka nui o ka pua puaʻa Kina, a ua hoʻololi ke ʻano mahiʻai mai ka hua kanu kuʻuna i ka moʻomeheu ulu a me ka transplantation. Eia nō naʻe, i ke kaʻina o ka moʻomeheu hua kanu a me ka hoʻololi ʻana, ʻoi aku ka nui o ka ulu ʻana o ka hypocotyl e hoʻohua i nā mea kanu leggy, e hopena i ka maikaʻi o ka hua. No laila, ʻo ka hoʻomalu ʻana i ka ulu ʻana o ka hypocotyl he mea koʻikoʻi ia i ka moʻomeheu mea kanu ikaika a me ka hoʻololi ʻana o ka kāpeti Kina. I kēia manawa, liʻiliʻi nā haʻawina e hoʻohui ana i ka transcriptomics a me ka ʻikepili metabolomics e ʻimi i ke ʻano o ka hypocotyl elongation. ʻAʻole i aʻo ʻia ke ʻano molekala e hoʻoponopono ai ka chlorantazole i ka hoʻonui ʻana o ka hypocotyl i loko o ka kāpeti Kina. Ua manaʻo mākou e ʻike i nā genes a me nā ala molekala e pane aku i ka uniconazole-induced hypocotyl dwarfing i loko o ka kāpena Kina. Ke hoʻohana nei i ka transcriptome a me ka metabolomic analyses, a me ka hū hoʻokahi-hybrid analysis, dual luciferase assay, a me ka virus-induced gene silencing (VIGS) assay, ua ʻike mākou ua hiki i ka uniconazole ke hoʻoulu i ka hypocotyl dwarfing i loko o ka kāpena Kina ma ke kāohi ʻana i ka biosynthesis lignin i nā hua kāpeti Kina. Hāʻawi kā mākou mau hualoaʻa i nā ʻike hou i ke ʻano hoʻoponopono molekala kahi e pale ai ka uniconazole i ka elongation hypocotyl i loko o ka kāpeti Kina ma o ka pale ʻana i ka biosynthesis phenylpropanoid i hoʻopili ʻia e ka module BrbZIP39-BrPAL4. Hiki i kēia mau hopena ke loaʻa nā manaʻo koʻikoʻi no ka hoʻomaikaʻi ʻana i ka maikaʻi o nā mea kanu kalepa a me ka hāʻawi ʻana i ka hōʻoia ʻana i ka hua a me ka maikaʻi o nā mea kanu.
Ua hoʻokomo ʻia ka BrbZIP39 ORF piha piha i loko o ka pGreenll 62-SK e hoʻohua i ka hopena, a ua hoʻohui ʻia ka ʻāpana mea hoʻolaha BrPAL4 i ka pGreenll 0800 luciferase (LUC) gene reporter e hoʻohua ai i ka gene reporter. Ua hoʻololi like ʻia ka mea hoʻoheheʻe a me ka mea hoʻolaha i nā lau paka (Nicotiana benthamiana).
No ka wehewehe ʻana i ka pilina o nā metabolites a me nā genes, ua hana mākou i kahi metabolome hui a me ka loiloi transcriptome. Ua hōʻike ʻia ka loiloi hoʻonui ʻana i ke ala KEGG ua hoʻohui pū ʻia nā DEG a me DAM ma nā ala 33 KEGG (Figure 5A). Ma waena o lākou,ʻo ke ala "phenylpropanoid biosynthesis" ka mea i hoʻonui nuiʻia; ʻO ke ala "photosynthetic carbon fixation", ke ala "flavonoid biosynthesis", ke ala "pentose-glucuronic acid interconversion", ke ala "tryptophan metabolism", a me ke ala "starch-sucrose metabolism" i hoʻonui nui ʻia. Ua hōʻike ʻia ka palapala ʻāina puʻupuʻu wela (Figure 5B) ua māhele ʻia nā DAM e pili ana me DEG i kekahi mau ʻāpana, a ʻo ia ka flavonoids ka māhele nui loa, e hōʻike ana i ke ala "phenylpropanoid biosynthesis" i hana koʻikoʻi i ka hypocotyl dwarfism.
Ua haʻi aku nā mea kākau ua hana ʻia ka noiʻi me ka loaʻa ʻole o nā pilina pāʻoihana a pili kālā paha i hiki ke manaʻo ʻia he paio o ka makemake.
ʻO nā manaʻo a pau i hōʻike ʻia ma kēia ʻatikala, ʻo ia wale nō ka manaʻo o ka mea kākau a ʻaʻole pono e hōʻike i ka manaʻo o nā hui pili, nā mea hoʻopuka, nā mea hoʻoponopono, a i ʻole nā mea loiloi. ʻO nā huahana i loiloi ʻia ma kēia ʻatikala a i ʻole nā koi i hana ʻia e kā lākou mea hana ʻaʻole i hōʻoia ʻia a ʻae ʻia e ka mea hoʻopuka.
Ka manawa hoʻouna: Mar-24-2025