Hoʻokomo ʻo Paclobutrazol i ka biosynthesis triterpenoid ma ke kāohi ʻana i ka mea hoʻoponopono transcriptional maikaʻi ʻole SlMYB i ka honeysuckle Iapana.

Loaʻa i nā halo nui ka waiwai a me nā ʻano like ʻole o nā metabolites bioactive a manaʻo ʻia he kumu waiwai waiwai. ʻO Phellinus igniarius kahi halo nui i hoʻohana mau ʻia no nā lāʻau lapaʻau a me nā kumu meaʻai, akā hoʻopaʻapaʻa ʻia kona helu ʻana a me ka inoa Latin. Ke hoʻohana nei i ka nānā ʻana i ka multigene segment alignment analysis, ua hōʻoia ka poʻe noiʻi ʻo Phellinus igniarius a me nā ʻano like ʻole no kahi genus hou a hoʻokumu i ka genus Sanghuangporus. ʻO ka mushroom ʻo Sanghuangporus lonicericola kekahi o nā ʻano Sanghuangporus i ʻike ʻia ma ka honua holoʻokoʻa. Ua hoʻokipa nui ʻia ʻo Phellinus igniarius ma muli o kāna mau lāʻau lapaʻau like ʻole, me nā polysaccharides, polyphenols, terpenes, a me nā flavonoids. ʻO Triterpenes nā pūhui pharmacologically ikaika o kēia genus, e hōʻike ana i nā hana antioxidant, antibacterial, a me nā hana antitumor.
Loaʻa i nā Triterpenoids ka mana nui no ka hoʻohana ʻana i ka ʻoihana. Ma muli o ka liʻiliʻi o nā kumu waiwai Sanghuangporus hihiu i ke ʻano, hoʻomaikaʻi maikaʻi i kona pono biosynthetic a me ka hua he mea koʻikoʻi. I kēia manawa, ua holomua ka hoʻonui ʻana i ka hana ʻana o nā metabolites lua o Sanghuangporus ma o ka hoʻohana ʻana i nā mea hoʻomohala kemika e kāohi i nā hoʻolālā fermentation submerged. No ka laʻana, ua hōʻike ʻia nā polyunsaturated fatty acids, fungal elicitors11 a me nā phytohormones (me ka methyl jasmonate a me ka salicylic acid14) e hoʻonui i ka hana triterpenoid ma Sanghuangporus. Nā mea hoʻoponopono ulu(PGRs)hiki ke hoʻoponopono i ka biosynthesis o nā metabolites lua i nā mea kanu. I loko o kēia noiʻi ʻana, ua noiʻi ʻia ʻo PBZ, kahi mea hoʻoponopono ulu mea kanu i hoʻohana nui ʻia e hoʻoponopono i ka ulu ʻana o ka mea kanu, ka hua, ka maikaʻi a me nā ʻano physiological. ʻO ka mea nui, hiki i ka hoʻohana ʻana i ka PBZ ke hoʻololi i ke ala biosynthetic terpenoid i nā mea kanu. ʻO ka hui pū ʻana o nā gibberellins me PBZ i hoʻonui i ka quinone methide triterpene (QT) ma Montevidia floribunda. Ua hoʻololi ʻia ke ʻano o ke ala terpenoid o ka ʻaila lavender ma hope o ka mālama ʻana me 400 ppm PBZ. Eia naʻe, ʻaʻohe hōʻike e pili ana i ka hoʻohana ʻana o PBZ i nā halo.
Ma kahi o nā haʻawina e pili ana i ka hoʻonui ʻana i ka hana triterpene, ua wehewehe kekahi mau haʻawina i nā ʻano hoʻoponopono o ka biosynthesis triterpene ma Moriformis ma lalo o ka mana o nā mea hoʻomohala kemika. I kēia manawa, ke nānā nei nā haʻawina i ka hoʻololi ʻana i nā pae hōʻike o nā genes structural e pili ana i ka biosynthesis triterpene i ke ala MVA, kahi e alakaʻi ai i ka hoʻonui ʻana i ka hana terpenoid.12,14 Eia naʻe, ʻo nā ala i lalo o kēia mau ʻano i ʻike ʻia, ʻo ia hoʻi nā mea transcription e hoʻoponopono ana i kā lākou ʻōlelo, ʻaʻole maopopo i nā ʻano hoʻoponopono o triterpenei biosyformis.
I loko o kēia noiʻi ʻana, ua noiʻi ʻia nā hopena o nā manaʻo like ʻole o nā mea hoʻoponopono ulu ulu (PGR) i ka hana triterpene a me ka ulu ʻana o ka mycelial i ka wā o ka fermentation submerged o ka honeysuckle (S. lonicericola). Ma hope mai, ua hoʻohana ʻia nā metabolomics a me nā transcriptomics e kālailai i ka haku mele triterpene a me nā hiʻohiʻona hōʻike gene i komo i ka biosynthesis triterpene i ka wā mālama PBZ. ʻO ka RNA-sequencing a me ka bioinformatics ʻike hou aʻe i ka helu transcription target o MYB (SlMYB). Eia kekahi, ua hoʻokumu ʻia nā mutants e hōʻoia i ka hopena hoʻoponopono o ka gene SlMYB ma ka biosynthesis triterpene a ʻike i nā genes i loaʻa. Ua hoʻohana ʻia ka Electrophoretic mobility shift assays (EMSA) e hōʻoia i ka pili ʻana o ka protein SlMYB me nā mea hoʻolaha o nā genes target SlMYB. I ka hōʻuluʻulu manaʻo, ʻo ka pahuhopu o kēia noiʻi ʻana ʻo ia ka hoʻoulu ʻana i ka biosynthesis triterpene me ka hoʻohana ʻana i ka PBZ a ʻike i kahi MYB transcription factor (SlMYB) e hoʻoponopono pololei i nā genes biosynthetic triterpene me MVD, IDI, a me FDPS ma S. lonicericola i pane i ka PBZ induction.
ʻO ka hoʻokomo ʻana o IAA a me PBZ i hoʻonui nui i ka hana triterpenoid i ka meli, akā ʻoi aku ka nui o ka hopena induction o PBZ. No laila, ua ʻike ʻia ʻo PBZ ʻo ia ka mea inducer maikaʻi loa ma kahi ʻoi aku ka nui o 100 mg / L, pono e aʻo hou.