Hoʻoulu ʻo Paclobutrazol i ka biosynthesis triterpenoid ma ke kāohi ʻana i ka mea hoʻoponopono transcriptional maikaʻi ʻole SlMYB i loko o ka honeysuckle Kepanī.

Loaʻa i nā halo nui kahi hui waiwai a ʻano like ʻole o nā metabolites bioactive a ua manaʻo ʻia he waiwai nui nā bioresources. ʻO Phellinus igniarius kahi halo nui i hoʻohana maʻamau ʻia no nā kumu lāʻau lapaʻau a me nā meaʻai, akā ʻo kona hoʻokaʻawale ʻana a me ka inoa Latina e hoʻopaʻapaʻa mau nei. Ma ka hoʻohana ʻana i ka loiloi hoʻonohonoho ʻāpana multigene, ua hōʻoia nā mea noiʻi ʻo Phellinus igniarius a me nā ʻano like no kahi ʻano hou a hoʻokumu i ka genus Sanghuangporus. ʻO ka halo honeysuckle Sanghuangporus lonicericola kekahi o nā ʻano Sanghuangporus i ʻike ʻia ma ka honua holoʻokoʻa. Ua huki nui ʻo Phellinus igniarius i ka nānā ʻana ma muli o kona mau waiwai lāʻau lapaʻau like ʻole, me nā polysaccharides, polyphenols, terpenes, a me flavonoids. ʻO Triterpenes nā hui pharmacologically active koʻikoʻi o kēia ʻano, e hōʻike ana i nā hana antioxidant, antibacterial, a me antitumor.
He nui ka hiki o nā Triterpenoids no ka hoʻohana ʻana i nā ʻoihana. Ma muli o ka laha ʻole o nā kumuwaiwai Sanghuangporus hihiu i ke kūlohelohe, he mea nui loa ka hoʻomaikaʻi pono ʻana i kona pono biosynthetic a me ka hua. I kēia manawa, ua holomua ka hoʻonui ʻana i ka hana ʻana o nā metabolites lua like ʻole o Sanghuangporus ma o ka hoʻohana ʻana i nā mea hoʻoulu kemika e kāohi i nā hoʻolālā fermentation i lalo. No ka laʻana, ua hōʻike ʻia nā waikawa momona polyunsaturated, nā elicitors fungal11 a me nā phytohormones (me ka methyl jasmonate a me ka salicylic acid14) e hoʻonui i ka hana triterpenoid ma Sanghuangporus. Nā mea hoʻoponopono ulu mea kanu(Nā PGR)hiki ke hoʻoponopono i ka biosynthesis o nā metabolites lua i nā mea kanu. Ma kēia haʻawina, ua noiʻi ʻia ʻo PBZ, kahi mea hoʻoponopono ulu mea kanu i hoʻohana nui ʻia e hoʻoponopono i ka ulu ʻana o nā mea kanu, ka hua, ka maikaʻi a me nā ʻano physiological. Ma ke ʻano kūikawā, hiki i ka hoʻohana ʻana o PBZ ke hoʻololi i ke ala biosynthetic terpenoid i nā mea kanu. ʻO ka hui pū ʻana o nā gibberellins me PBZ i hoʻonui i ka ʻike quinone methide triterpene (QT) ma Montevidia floribunda. Ua hoʻololi ʻia ke ʻano o ke ala terpenoid o ka ʻaila lavender ma hope o ka mālama ʻana me 400 ppm PBZ. Eia nō naʻe, ʻaʻohe hōʻike e pili ana i ka hoʻopili ʻana o PBZ i nā halo.
Ma waho aʻe o nā haʻawina e kālele ana i ka hoʻonui ʻia ʻana o ka hana triterpene, ua wehewehe pū kekahi mau haʻawina i nā ʻano hoʻoponopono o ka biosynthesis triterpene ma Moriformis ma lalo o ka mana o nā mea hoʻoulu kemika. I kēia manawa, ke kālele nei nā haʻawina i ka hoʻololi ʻana o nā pae hōʻike o nā genes structural e pili ana i ka biosynthesis triterpene ma ke ala MVA, kahi e alakaʻi ai i ka hoʻonui ʻia ʻana o ka hana terpenoid.12,14 Eia nō naʻe, ʻaʻole maopopo nā ala e pili ana i kēia mau genes structural i ʻike ʻia, ʻoiai nā kumu transcription e hoʻoponopono ana i kā lākou hōʻike ʻana, i nā ʻano hoʻoponopono o ka biosynthesis triterpene ma Moriformis.
Ma kēia haʻawina, ua noiʻi ʻia nā hopena o nā ʻano like ʻole o nā mea hoʻoponopono ulu mea kanu (PGR) ma ka hana triterpene a me ka ulu ʻana o ka mycelial i ka wā o ka fermentation submerged o ka honeysuckle (S. lonicericola). Ma hope mai, ua hoʻohana ʻia nā metabolomics a me nā transcriptomics e kālailai i ka haku mele triterpene a me nā ʻano hōʻike gene i komo i ka biosynthesis triterpene i ka wā o ka mālama ʻana iā PBZ. Ua ʻike hou aku ka ʻikepili RNA-sequencing a me bioinformatics i ka target transcription factor o MYB (SlMYB). Eia kekahi, ua hana ʻia nā mutants e hōʻoia i ka hopena hoʻoponopono o ka gene SlMYB ma ka biosynthesis triterpene a ʻike i nā genes target hiki. Ua hoʻohana ʻia nā Electrophoretic mobility shift assays (EMSA) e hōʻoia i ka launa pū ʻana o ka protein SlMYB me nā mea hoʻolaha o nā genes target SlMYB. I ka hōʻuluʻulu manaʻo, ʻo ka pahuhopu o kēia haʻawina ʻo ia ke hoʻoulu i ka biosynthesis triterpene me ka hoʻohana ʻana iā PBZ a ʻike i kahi MYB transcription factor (SlMYB) e hoʻoponopono pololei i nā genes biosynthetic triterpene e pili ana iā MVD, IDI, a me FDPS ma S. lonicericola i ka pane ʻana i ka hoʻokomo ʻana o PBZ.
Ua hoʻonui nui ka hoʻokomo ʻana o IAA a me PBZ i ka hana triterpenoid i loko o ka honeysuckle, akā ua ʻoi aku ka ʻike ʻia o ka hopena hoʻokomo o PBZ. No laila, ua ʻike ʻia ʻo PBZ ka mea hoʻoulu maikaʻi loa ma kahi hoʻohui hou o 100 mg/L, kahi mea e pono ai ke aʻo hou ʻia.